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With advancing age, structural and functional perturbations within this organelle interaction network lead to convergent pathological outcomes, including impaired energy supply, excessive ROS accumulation, Ca 2+ signaling imbalance, and defective autophagy and quality control

Food intake was not impacted, and any observable side effects or toxicity were negligible to the point where they should not be taken seriously

Autolytic activation and disulfide-mediated dimerisation of neprosin Mature Clc and Np were obtained by autolytic activation of the zymogens, following the protocol established for pNp (del Amo-Maestro et al, 2022)

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Following the panning procedure, 100 l of the eluted solution was added on the RMG plates (1 M9 salt, 0.2% casamino acid, 0.5% glucose, 1 mM MgCl 2 , 1.5% agar)