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Add 1 mL of Reagent I (the proportion of tissue and reagents can be kept constant), fully grinding on ice (using liquid nitrogen will have a better grinding effect)

The absorbance at 570 nm was measured using a microplate reader (Epoch, BioTek, US)

Future collaborations between academics, clinicians and milk banks, especially in low-income countries, should be fostered for greater access to the potential applications of human milk

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Concomitant with these results, immunofluorescence for -smooth muscle actin (SMA) indicated reduced presence of hepatic stellate cells, the main cells driving fibrogenesis 3,51 , in livers from mice treated with AAV8-AGXT (Fig