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Metabolic fingerprints (e.g., OCR/ECAR ratio, mtDNA copy number) are emerging as key indicators for assessing resistance risk [176, 177]alongside KRAS and BRAF mutation status

study also found that TAC measured by ABTS correlated negatively with age and positively with FEV1

The ATP-grasp fold is conserved within the ATP-grasp superfamily and is characterized by two alpha helices and beta sheets that hold onto the ATP molecule between them

Briefly, for mitochondria isolation, cells were resuspended in an equal volume ice-cold hypotonic buffer (10 mM NaCl, 1.5 mM MgCl 2 , 10 mM HEPES, pH 7.5) and allowed to swell for 10 minutes

Notably, TDLNs are enriched for tumor-specific PD-1+ T cells, which frequently interact with PD-L1+ conventional dendritic cells ( Figure 3 )