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Importantly, NAD+ is only one part of a complex biological system

Incubations were performed at 37 C with agar plates placed in an atmosphere of 5% CO 2 and broth cultures aerobically shaken at 170 rpm

It is unique in being encoded by mitochondrial rather than nuclear DNA

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The reversible nature of the JNK1 1a R -IN-8 complex, in contrast to the irreversible JNK1JNK-IN-8 complex, was also confirmed by these experiments since some enzymatic activity of JNK1 ( ~ 50%) could be regained if the enzyme had bound to 1a R -IN-8 but not when to JNK-IN-8