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In the current study, following manufacturers instruction, 20 L from each sample, 100 L of calibrator and 100 L assay buffer were transferred to separate wells in a Nunc 96-well polypropylene MicroWell plate (Thermo Scientific Nunc, Roskilde, Denmark)

LPS activated microglial cells had upregulated levels of oxidative stress markers, such as NO, superoxide anion and malondialdehyde (MDA), while also upregulating antioxidative defense system enzymes involved in antioxidative defense and glutathione, non-enzymatic antioxidant

5-Amino-1MQ (NNMT Inhibitor Compound) is a small-molecule research compound