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Abcam) were added dropwise, incubated for 2 h at room temperature and then rinsed 5 times for 2 min each using PBS buffer

Other reagents for peptide synthesis and cleavage, including GSH, dichloromethane (DCM), N, N-dimethylformamide (DMF), acetonitrile, Tris-(2-carboxyethyl) phosphine (TCEP), triisopropylsilane (TIPS), N, N-diisopropylethylamine (DIEA), p-cresol and guanidine hydrochloride (GuHCl) were purchased from Sigma-Aldrich

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The mitochondria in syncytiotrophoblasts (STBs) are responsible for placental steroid hormone production, especially progesterone [10]

However, always consult a healthcare provider before use