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Structural insights into the quaternary catalytic mechanism of hexameric human quinolinate phosphoribosyltransferase, a key enzyme in de novo NAD biosynthesis

J Mater Chem B Royal Soc Chem (RSC)

2.14 Co-immunoprecipitation To perform co-immunoprecipitation, PC12 cells (CRL-1721) were lysed in a buffer containing 50 mM Tris (pH 7.5), 150 mM NaCl, 2 mM EGTA, 0.1% Triton X-100, 2 mM MgCl 2 , 2 mM MgATP, 50 mM NaF, and 1 mM Na 3 VO 4, supplemented with the cOmplete Protease Inhibitor Cocktail and phosphatase inhibitor PhosSTOP

Both PENDRIN and NIS were expressed on the surface of human leukocytes

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