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Changes of the glutathione redox system during the weaning transition in piglets, in relation to small intestinal morphology and barrier function

Feb 3 2006;340(1):291-295

Generation and quantification of protein persulfides and polysulfides in cell lysates HEK 293 cell lysate (200 g) protein/sample in 50 mM HEPES buffer (pH 7.4) was treated with Na 2 S (040 M) or Na 2 S 2 (0600 M) for 7 min in sealed Eppendorf tubes with minimal head space at RT