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Global metabolic profiling and relative quantification for steady-state analyses were performed using a HILIC assay specialized for broad detection and high resolution of polar metabolites (energy metabolism and other central carbon metabolites) with the AdvanceBio Glycan Map 2.1 100 mm 2.7 m column with Infinity II in-line filter (Agilent Technologies, Wilmington, DE, USA)

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Beyond tumorintrinsic effects, lactylation also modulates immune cell function directly, for example, H3K18la upregulates the immunosuppressive molecule B7H3 in CD8 + T cells, inducing T cell exhaustion (78, 79)

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