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On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin

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This product is manufactured with a purity level of 99% or higher for reliable research use