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Role of histone deacetylases and their inhibitors in neurological diseases
While studies attempting to localize the pathology in FMS to peripheral tissues have failed in showing any abnormalities in muscle tissue, Staud and coworkers did show that enhanced central pain processing can occur via continued peripheral muscle afferent input, Their study demonstrated that muscle lidocaine injections increased local pain thresholds and decreased remote secondary heat hyperalgesia in fibromyalgia patients, emphasizing the important role of peripheral impulse input in maintaining central sensitization
Liver slices (thickness: 200 300 m) were prepared in ice-cold KrebsHenseleit buffer, pH 7.42, supplemented with 25 mM NaHCO 3 , 25 mM D-glucose, 10 mM 2-(4-(2-Hydroxyethyl)- 1-piperazinyl)-ethanesulfonic acid (HEPES) (all from Merck, Darmstadt, Germany) and saturated with carbogen (95% O 2 , 5% CO 2 ) using a Krumdieck tissue slicer (Alabama Research and Development, Munford, AL, USA)
Delivered reconstituted, not in powdered form as seen when sourced from a lab
