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The low GSH concentration necessary for CVA21 displacement may also limit a co-elution of potentially bound host cell GST enzymes present in cell culture harvests, which may require as high as 10 mM GSH for elution (Figure 4A) and elution NaCl gradient (Supplementary Figure S6C) studies where CVA21 particles eluted at lower ionic strengths with increasing pH

Sample preparation for FACS analysis of individually cultured clones was performed essentially as described above, applying both Pap and NH 2 -Pap at different concentrations

Environmental insults: critical triggers for amyotrophic lateral sclerosis

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Similarly, PD pathogenesis involves multiple pathways, including -synuclein aggregation, mitochondrial dysfunction, oxidative stress, calcium dysregulation, axonal transport defects, and neuroinflammation