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10.1016/S0022-3956(03)00022-0 77 UhrM.TontschA.NamendorfC.RipkeS.LucaeS.IsingM.et al (2008)

Both genes were genotyped using a 25-L reaction mixture containing 1 L of genomic DNA template (100 ng/L), 1 L of each primer (20 pmol/mL), 0.5 L of dNTPs (200 M), 2.5 L of PCR buffer with 15 mM/L MgCl 2 , and 0.5 L of Taq polymerase (3 U/L) and 19.5 L sterile nuclease free water

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Plasma markers in pulmonary hypertension subgroups correlate with patient survival