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We measured OCR in cells either pre-conditioned, or not, with Exendin-4 after injection of glucose, oligomycin, FCCP and a combination of rotenone and antimycin A (Fig

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The cumulative error becomes catastrophic to your data's validity

doi: 10.1038/nrm3028 146 BattagliaAMChirilloRAversaISaccoACostanzoFBiamonteF

however, the accumulated excess intracellular cystine induces disulfide stress, leading to the overproduction of ROS and gradually promoting ferroptosis [16]