Folate is an essential vitamin that participates in enzymatic reactions involved in the one-carbon metabolic pathways, regulating DNA methylation, and amino acid and lipid metabolism (95)
Cytochrome cs terminal oxidation is mediated by cytochrome c oxidase through molecular oxygen (cox or complex 1V) (Watson and McStay, 2020)

Abbreviations CSE: Cigarette smoke extract TNF-: Tumor necrosis factor- EMT: Epithelial-mesenchymal transformation COPD: Chronic obstructive pulmonary disease CS: Cigarette smoke NAC: N -Acetylcysteine VWF: Von Willebrand factor MAPK: Mitogen-activated protein kinase FEV1: Forced expiratory volume in 1 FVC: Forced vital capacity PMSF: Phenylmethylsulphonyl fluoride ECL: Enhanced chemiluminescence SABC: Streptavidin biotin peroxidase complex ACK: Ammonium chloride-potassium FITC: Fluorescein isothiocyanate NC: Negative control DMEM: Dulbecco's modified Eagle's medium oe: Overexpression sh: Short hairpin RNA SD: SpragueDawley BCA: Bicinchoninic acid PEF: Peak expiratory flow HE: Hematoxylineosin RT-qPCR: Reverse transcription quantitative polymerase chain reaction GAPDH: Glyceraldehyde-3-phosphate dehydrogenase RIPA: Radio-immunoprecipitation assay SDS-PAGE: Sodium dodecyl sulfatepolyacrylamide gel electrophoresis PVDF: Polyvinylidene fluoride ANOVA: Analysis of variance References Armstrong J, Harbron C, Lea S, Booth G, Cadden P, Wreggett KA, et al

ensure adequate iron, folate, vitamin D, zinc, magnesium, and iodine
L-glutathione is the bodys antioxidant that is produced naturally by the body.Yet, the bodys ability to make L-glutathione tends to decline with advancing age