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Description

75-100g of protein lysates were mixed with Laemmli 6x SDS sample buffer (Boston BioProducts, BP-111R) with 5% 2-mercaptoethanol (VWR Life Science M131) and then ran on 4-20% Criterion TGX pre-cast gels (Bio-Rad 5671093)

It was found in multiple tumor cell lines that hypermethylation of the GPX3 promoter CpG island resulted in downregulation or complete silencing of GPX3 expression [40]