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Primary neuron cultures In preparation for primary neuron harvests, glass-bottom chamber slides were coated with 0.5 mg/mL poly-D-lysine (Sigma, P7886-100MG) in borate buffer [12.5 mM sodium borate decahydrate (VWR, MK745706) and 50 mM boric acid (Sigma, B6768-1KG)] overnight at room temperature, then washed 4x with sterile deionized water

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The cells were then fixed with 4% paraformalde-hyde (cat

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doi: 10.1016/j.snb.2019.126646