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Evidence that metformin exerts its anti-diabetic effects through inhibition of complex 1 of the mitochondrial respiratory chain

A2780 and A2780DPP cells (2.5 10 5 cells per well in six-well plates) were transfected with each plasmid using lipofectamine (Life Technologies, Paisley, UK), and shRNA-containing cells selected with puromycin

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Furthermore, we found that the expression of the p-NF-B p65 protein was upregulated in peroxynitrite-exposed SGNs compared with the untreated controls, and the pretreatment with ebselen significantly reduced it while the lack of GPX1 increased it (Figure 8C)

The lungs (excluding the heart) were carefully removed and incubated in 5 mL of digestive solution in a 15 mL tube for 6090 min in a 37 C water bath