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10.1371/journal.pone.0063197 PLoS One

This prevents the formation of aggregates which could interfere with spectrophotometric analysis or other analytical techniques

In this context, in describing early reporting on the use of human subjects for mustard research in 1919, Sollman explained that experiments on animals was [sic] abandoned after a few trials, since their skin does not react in the same manner as human skin, and the effects that do occur are not easily graded. Reference Sollman8 Blistering is not commonly observed in animals