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This is due in part to the use of fluorescent resonance energy transfer (FRET), fluorophores were carefully selected such that, when the fluorophores are paired, activation of the donor fluorophore causes a highly efficient energy transfer to the acceptor fluorophore, producing a large emission from the acceptor fluorophore, this effect diminishes exponentially as the distance between the fluorophores increases

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however, recent research has indicated that autophagy selectively eliminates specific components, referred to as selective autophagy, including peroxisomes (pexophagy), mitochondria (mitophagy), lysosomes (lysophagy), and ER (reticulophagy) 52

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Eur.J Gastroenterol.Hepatol