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Melting curves were fitted in Origin Pro 9 software using the Boltzmann sigmoidal equation F = F min + ( F max F min )/(1 + exp(( T t T )/ s )), where F refers to fluorescence ( = 660 nm) and F min and F max are the minimum and maximum fluorescence, respectively, T is temperature, T t is the transition temperature, and s is a fitting parameter

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SIRT3 promotes lipophagy and chaperon-mediated autophagy to protect hepatocytes against lipotoxicity

Watras MM, Patel JP, Arya R