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3.3.3 Screening of the anti-cancer drugs targeting breast cancer stem cells Cancer stem cells (CSCs) possess three defining hallmarks: (1) self-renewal capacity, (2) multilineage differentiation potential, and (3) tumor-initiating ability, making them prime therapeutic targets for eradicating malignant clones

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Common IF patterns include Every-other-day fasting (EODF), whole-day fasting (1 or 2 days of fasting per week), time-restricted feeding (TRF, where energy intake is typically limited to an 812 h window), and periodic fasting (PF, fasting for 2 days following 5 days of unrestricted eating) (29) (Figure 1)