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Furthermore, a different approach using intragastrically administered rotenone (5 mg/Kg) in mice showed that the accumulation and aggregation of -synuclein in neurons of the dorsal motor nucleus of the vagus (DMV) and the intermediolateral nucleus (IML) in the spinal cord was accompanied by the selective loss of dopaminergic neurons and astrogliosis, suggesting that the gastric administration of rotenone through the connection of the enteric nervous system (ENS) with anatomical structures of the CNS also induces PD-like features [11,19]

All primary antibodies were diluted with PBS-0.05% Tween 20 (PBST), and added onto precoated ELISA plates with incubation at 37C for 2 h

This piece covers the actual pharmacokinetic differences, how injection technique affects outcomes, and what preparation errors negate the benefits of either route

PMID 9610496

These results indicate that -glutamylcysteine is an important player in cellular redox control