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If the OD value at 620nm is lower than 0.8, the staining duration can be properly extended

Notes All antibodies are custom made on-demand

GEFs activate GTPases by promoting the exchange of bound GDP-for-GTP while GAPs inhibit GTPases by hydrolyzing bound GTP-to-GDP

These Phase II conjugates can be thought of as having chemical labeling tags which notify cells that the molecule is to be disposed of

Here, we employed comparative global metabolomics to demonstrate that metabolism of the antioxidant glutathione (GSH) is inextricably linked to redox-active processes that facilitate melanin production, and that genetic perturbations in GSH biosynthesis affect fungal growth and virulence in a murine model of cryptococcosis