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For example, the redox activitiy of p66Shc within mitochondria has been shown to generate H 2 O 2 in the absence of superoxide through oxidation of cytochrome c (Giorgio et al., 2005)

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After the imaging, the cells were trypsinised, re-suspended in the complete media, and relative number of living cells were counted by using Countess II after staining with trypan blue

Most importantly, it has been shown that dietary vitamin C supplementation does not significantly affect ASL concentrations of ascorbic acid [79, 80]