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Under the condition of exogenous NPY overexpression, bone formation and bone microarchitecture were disrupted, pyroptosis-related molecules such as NOD-like receptor protein 3 (NLRP3) and Caspase-1 in subchondral cancellous bone were upregulated, and the levels of colonic inflammatory indicators like IL-1, IL-18, and serum LPS were elevated as well

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They first blocked blood flow to simulate a major ischemic stroke and then restored it (a process called reperfusion), just as doctors restore blood flow acutely for ischemic stroke patients