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First, the designed approach needs to be internally validated on the discovery dataset it was based upon, using appropriate internal validation procedures such as k-fold cross-validation

Despite this extra CBM loop, the absence of a loop ~15 residues in length that resides at the Loop 1 position may explain why Bu GUS, Bd GUS, and other non-L1 GUS enzymes exhibited higher K m values for p NPG compared to Ec GUS, Ee GUS, Sa GUS, and Cp GUS

*Correspondence: Hao Jiang, [email protected] Kim, [email protected] These authors have contributed equally to this work This article was submitted to Cell Growth and Division, a section of the journal Frontiers in Cell and Developmental Biology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

ACS Chem Biol (2016) 11(5):130512

Previous studies have explored the mechanisms of iron-induced cell death in mice and found that iron overload can lead to ferroptosis