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Description

To prepare paraffin sections, the fixed brain tissue was dehydrated with 70, 80, 95 and 100% alcohol followed by transparent with xylene, embedded in paraffin and cut into 5 m thickness with a microtome (Leica, Germany)

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Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation [8]

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Human Data: Endogenous plasma levels of MOTS-c are observed to increase following human exercise, linking it directly to exercise biology

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