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These samples then were analysed on ICP-AES (Inductively Coupled Plasma Atomic Emission Spectroscopy) for macro-element and trace elements

Intracellular components are first engulfed in double membrane vesicles called autophagosomes, which subsequently fuse with lysosomes for digestion of their contents and recycling of their building blocks

Nickel nanoparticles affect the migration and invasion of HTR-8/SVneo cells by downregulating MMP2 through the PI3K/AKT pathway

In case of Drosophila , neuronal redox balance was altered by driving CatalaseRNAi or PHGpxRNAi, along with roGFP by elav-Gal4 to deplete the respective antioxidant enzymes and express the redox biosensor

Wash with PBS one more time and add 500 l of lysis buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5) on ice