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10.1038/s41589-019-0291-9 108 TsvetkovP.CoyS.PetrovaB.DreishpoonM.VermaA.AbdusamadM.et al (2022)

In addition, the Ser558 site in the Neh1 domain of Nrf2 has been identified as a direct phosphorylation site of AMPK (Joo et al., 2016), which results in Nrf2 activation via the Ca 2+ -CaMKK-AMPK pathway

Freshly dissected lenses were lysed in NP40 lysis buffer (Life Technologies) with 1 mM PMSF and protease inhibitor cocktail (Complete, Roche) using the Qiagen TissueLyser II