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The top phase was transferred to a scintillation vial for counting of the fatty acid fraction using a TopCount NXT Microplate Scintillation and Luminescence Counter (Perkin Elmer)

The direct comparison upon using the same concentration of ruthenium of the molecular agent Ru(II)-OH and NP4 showed a significantly higher tumor accumulation of the nanoparticle formulation NP4 (Supplementary Fig