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doi: 10.1016/s0304-3835(03)00480-4

After washing three times with TBST, the membranes were incubated with HRP-conjugated secondary antibody (Amersham Biosciences) in blocking buffer

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In this study, we sought to investigate the role of cystine in the regulation of mTORC1, the ISR and ferroptosis and whether GSH is an important determinant in the regulation independent of its reducing capacity