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Unfortunately, there is limited evidence to support the above options as being the optimal way to increase glutathione

However, the stability and reproducibility of metabolomics biomarkers in different populations, as well as interactions between metabolites and other biological factors need to be further clarified

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Detection of mitochondrial ROS, apoptosis, and viability For mitochondrial ROS measurements, cells were stained with MitoSOX Red (2.5 M, 30 minutes, dark), washed with phosphate-buffered saline (PBS), detached using trypsin-EDTA, and washed twice in Hanks Balanced Salt Solution (HBSS) by centrifugation (5 min, 1200 rpm, room temperature)