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After centrifugation, the supernatant was passed through a 0.45 m filter and proteins were purified by affinity chromatography using NEBExpress Ni resin (New England Biolabs, Frankfurt, Germany) with increasing imidazole concentrations of 50 to 300 mM

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Of those 7,604 cases, there were 579 cases reporting an aortic valve repair/replacement procedure, a mitral valve repair/replacement procedure, and another concomitant procedure with average costs of $85,890 and an average length of stay of 13.7 days

Further, optical densities were measured at 570 nm with a SpectraMax M2 microplate reader (Molecular Devices, USA)

doi: 10.1038/s41598-024-83835-0 48 LpezPde PazBRodrguez-CarrioJHeviaASnchezBMargollesAet al