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coli BL21(DE3) cys auxotrophic strain 23,24,25 following the previously described protocol with slight modifications 16,26

Proc Natl Acad Sci U S A 107:59835988 Kabsch W (2010) XDS

in the mRNA CDS region forms a 'ribosomal speed bump' that enhances the translation efficiency of rare codons through prolonging the ribosomal retention time, whereas the -modification of termination codons (namely, changing UGA to GA) results in an inhibition of the recognition of transcripts by the eukaryotic release factor eRF1, resulting in an 15% prolongation of readthrough translation (132,133)

For acute injuries with significant pain and swelling, some practitioners and experienced users suggest a higher loading approach during the first 3-5 days: 0.5 mg twice daily (1 mg total) before reducing to maintenance dosing