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SHBG decreases the inflammation and enhances the anti-inflammatory properties of EMS affected EqASCs In order to determine the anti-inflammatory activity of the SHBG application on the EqASC EMS , we analyzed the main important inflammatory ( IL-1 , IL-6 and TNF- ) and anti-inflammatory markers ( IL-4 , IL-10 and IL-13 ) via RT-qPCR for the gene expression analysis as well as the western blot for proteins expression analysis (Fig