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I outlined this in the Peptides deep dive in the Missing Context Newsletter

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The cell powder was resuspended with lysis buffer (20 mM HEPESKOH pH 7.4, 100 mM KOAc, 2 mM Mg(OAc) 2 , 0.5 mM DTT, 1 mM PMSF and one tablet/10 mL complete mini EDTA-free (Roche)) to prepare the crude extracts