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As Marre says if the fluid was cold then it would sting a lot as well - they tend to store ampoules in the fridge so they are way off body temperature unless they are warmed in the hands first

LOCF is of some statistical value to determine efficacy, but it is of limited value if a real-life treatment-effect size is required

Placebo effects in low back pain: A systematic review and metaanalysis of the literature

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However, elite sports organizations that follow WADA guidelines may prohibit any substance that enhances NAD or mimics metabolic regulation