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In addition, the EGFP expression level determined by RT-qPCR also confirmed the enrichment efficiency of the cell sorting operation, with five times for pcDNA3.1-GST DAF -ATP6AP1L and 3.3 times for pcDNA3.1-GST CEAM7 -ATP6AP1L (Figure 6C)

Icariside I improves the microbiota community structure, alleviates inflammation, promotes the restoration of the intestinal barrier, and has significant antitumor effects [191]