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The clear supernatant was separated off within 2 h and the cholesterol content was determined by the CHOD-PAP reaction method as follows: One milliliter cholesterol reagent was added separately to 100 L each of serum and organs homogenates and mixed together in a test tube

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Other factors such as genetic predisposition, allergies, psychological stress, and microbiota also play a role in the occurrence and development of AA (2)

Nutrition (2012) 28:53943

Because IDO1 -associated inflammatory activation would be expected to increase, rather than decrease, the Kyn/Trp ratio [46,47,48], a one-tailed analysis was also performed