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Using standardized reconstitution volumes simplifies the math and ensures consistency across the protocol

Finally, soluble nuclear lysate was diluted with two volumes of the dilution buffer (60 mM HEPES-KOH (pH = 7.9), 1 mM DTT, 30 % glycerol, 0.4 % NP-40, 1 x phosphatase inhibitor cocktail (Sigma, P0044) and 1 x protease inhibitor cocktail (Sigma, P8340)) to lower KCL concentration to 150 mM

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Figure 2 3.3.1 NLRP3 inflammasome pathway NOD-like receptor protein (NLRP)3 is a multiprotein complex that functions to activate IL-1 (141)