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The absorbance at 450 nm was measured using a microplate reader (ELX800, BioTek Instruments Inc., Winooski, VT, USA) to determine cell viability relative to the control group

Paoli, L

Proinflammatory cytokine regulation The total RNA was extracted from the right ankle joint tissues for determining the expressions of mRNAs of IL-6, IL-1 and TNF- by RT-PCR

Northern blot analysis Total RNA was extracted from roots and shoots, and 10 g were electrophoresed in 1.2% (w/v) agarose gels containing 0.66 M formaldehyde and transferred to Hybond-N+ membranes (Amersham, Piscataway, NJ)