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Description

This work emphasizes the importance of metabolic competition in the tumor microenvironment

Proteins were separated by electrophoresis under reducing conditions on a 420% Criterion Tris-Glycine protein gel (BioRad #3450033), transferred to a 0.2 m nitrocellulose membrane (BioRad #1620112), and then blocked for 90 min in 5% skim milk in TBS-Tween 20