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For the non-interventional cohort, a group size of 20 subjects will detect a minimum difference in baseline GSH of at least 16% between young and elderly healthy subjects (0.05 alpha, 0.8 power) based on an inter-subject CV of 22%

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The sample was digested in an oven at 60 C using 0.1 ml trace-metal-grade concentrated HNO 3 (69% w/w, respectively