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Both conditions (treatment and control) were analyzed in 3 biological triplicates for each cell line

Social preference was quantified as an interaction ratio: time in the novel-conspecific chamber divided by the total time in both chambers

The same principle probably applies also to the elicitation of allergic reactions in sensitized individuals

It incorporates key modifications, including 14E/17R mutations to stabilize an -helical segment via a salt bridge, multiple proline substitutions (25P, 28P, 29P) to reduce -sheet formation, and N-terminal C20 fatty diacid lipidation for reversible albumin binding (Kruse et al