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An alternative approach is to remove the erythrocytes from whole blood first by aggregation with dextran [2-4], methylcellulose [5,6] hetastarch [7] or Plasmagel [8,9] and then layer the resulting leukocyte-rich plasma (LRP) over the Nycoprep 1.077A, so that the PMN pellet contains only a very small percentage of residual erythrocytes