glutathione estimation method assay calculation Full article: Comparison of three methods for in vivo quantification of in tissues of hypertensive rats Glutathione Quantification in Live Cells – estimation of glutathione peroxidase activity

Samples were mixed with a nondenaturing PAGE loading buffer containing 5% (v/v) glycerol, bromophenol blue and Trisborate (TB) buffer (100 mM Tris and 100 mM boric acid) and run on a native PAGE gel (prerun at 200 V for 5 min) for 50 min (130 V, 4 C) in TB buffer

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Rocca C, Bartolo AD, Granieri MC, Rago V, Amelio D, Falbo F, Malivindi R, Mazza R, Cerra MC, Boukhzar L, Lefranc B, Leprince J, Anouar Y, Angelone T (2022) The antioxidant selenoprotein T mimetic, PSELT, induces preconditioning-like myocardial protection by relieving endoplasmic-reticulum stress

I would respectfully ask if the B12 test could be repeated, I have learned here that B12 supplementing can alter folate levels so I wonder if your folate level being considerably above the high end of the range has masked your B12 deficiency and affected your B12 levels