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1,4,21,22 However, it is worth mentioning that some studies reported pain at the site of injection and knee pain which could have been induced by an overload of volume due to lack of absorption by the damaged meniscus

The membrane was blocked in blocking buffer (5% BSA in 1TBST, Tris-buffered saline and Tween-20) and then incubated with primary antibodies overnight at 4 C

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