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Agudelo et al

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The obtained cell pellet was lysed with 5% SDS + 0.1 M Tris buffer in a 1:1 ratio to minimize proteolysis and kept at 80 C until further analysis

demonstrated that NAC administration significantly attenuated acute lung injury in septic rats by reducing oxidative stress, inflammatory cytokine production, and apoptosis (Le et al., 2022)