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Cultures were grown until they reached the exponential growth phase (OD 600 = 0.5), filtered through a 5 m diameter pore membrane (Millipore, Inc., Billerica, MA) to remove cell clusters, and then centrifuged and resuspended in fresh media to obtain a single-cell suspension at OD 600 = 0.01

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An update to the 2007 review in Annals of Botany

The cell cycle inhibitor, CDKN2D /p19 INK4d , and NFT molecular signatures significantly overlapped with the senescent neuron population (39)

CBS is responsible for the production of cystathionine, cysteine and H 2 S in the transsulfuration pathway, all of which are key antioxidant molecules 36